Anticorps Polyclonal de lapin anti-RAPGEF5

RAPGEF5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, RIP, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 12556-1-AP

Synonymes

GFR, KIAA0277, M Ras regulated Rap GEF, MR GEF, MRGEF, RAPGEF5, Related to Epac, REPAC

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Galerie de données de validation

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  WB analysis of human brain using 12556-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12556-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12556-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12556-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12556-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12556-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HEK-293, cellules HeLa, cellules K-562, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

12556-1-AP cible RAPGEF5 dans les applications de WB, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAPGEF5 Protéine recombinante Ag3229
• Nom complet: Rap guanine nucleotide exchange factor (GEF) 5
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 68-75 kDa
• Numéro d’acquisition GenBank: BC039203
• Symbole du gène: RAPGEF5
• Identification du gène (NCBI): 9771
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,RIP	Redox Biol; CircRAPGEF5 interacts with RBFOX2 to confer ferroptosis resistance by modulating alternative splicing of TFRC in endometrial cancer; Authors - Jun Zhang