Anticorps Polyclonal de lapin anti-EPAC2

EPAC2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 19103-1-AP

Synonymes

CAMP GEFII, CGEF2, EPAC 2, EPAC2, Nbla00496, RAPGEF4

---

Galerie de données de validation

• 

  WB analysis of mouse brain using 19103-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19103-1-AP (EPAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 19103-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19103-1-AP (EPAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19103-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19103-1-AP (EPAC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 19103-1-AP

  IP result of anti-EPAC2 (IP:19103-1-AP, 4ug; Detection:19103-1-AP 1:600) with mouse brain tissue lysate 2560ug.

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HepG2, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

19103-1-AP cible EPAC2 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EPAC2 Protéine recombinante Ag5186
• Nom complet: Rap guanine nucleotide exchange factor (GEF) 4
• Masse moléculaire calculée: 116 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC024004
• Symbole du gène: EPAC2/RAPGEF4
• Identification du gène (NCBI): 11069
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB	J Cell Mol Med; Differential changes in cyclic adenosine 3'-5' monophosphate (cAMP) effectors and major Ca2+ handling proteins during diabetic cardiomyopathy; Authors - Victoria Chaoul
rat	WB,IF	J Diabetes Investig; Epac2 activation mediates glucagon-induced glucogenesis in primary rat hepatocytes; Authors - Yusuke Shiozaki-Takagi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tongbin (Verified Customer) (08-25-2020)

This EPAC2 antibody successfully detected a strong EPAC2 band in mouse heart lysate, which is about 120 kDa.

• Applications: Western Blot
• Cell Tissue Type: Heart