Anticorps Polyclonal de lapin anti-RAP2B

RAP2B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 16266-1-AP

Synonymes

RAP2B, Ras related protein Rap 2b

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Galerie de données de validation

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  WB analysis using 16266-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16266-1-AP (RAP2B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16266-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16266-1-AP (RAP2B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 16266-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16266-1-AP (RAP2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16266-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16266-1-AP (RAP2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, cellules MCF-7, tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

16266-1-AP cible RAP2B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAP2B Protéine recombinante Ag9331
• Nom complet: RAP2B, member of RAS oncogene family
• Masse moléculaire calculée: 183 aa, 21 kDa
• Poids moléculaire observé: 19-22 kDa
• Numéro d’acquisition GenBank: BC012362
• Symbole du gène: RAP2B
• Identification du gène (NCBI): 5912
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAP2B belongs to a family of RAS-related genes. It share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. Rap2B is a platelet protein that is activated by thrombin and involved in platelet activation. RAP2B may be a novel candidate oncogene that plays important roles in carcinogenesis through activation of NF-kappaB pathway.

Publications

Species	Application	Title
mouse	WB	J Biol Chem; DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.; Authors - Li Yi Y