Anticorps Polyclonal de lapin anti-RAMP3

RAMP3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 11861-1-AP

Synonymes

RAMP3

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Galerie de données de validation

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  WB analysis using 11861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11861-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11861-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11861-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11861-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11861-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11861-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11861-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11861-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11861-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11861-1-AP (RAMP3 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Jurkat, cellules MCF-7, tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral de souris, tissu de muscle squelettique de souris, tissu pancréatique de souris, tissu placentaire humain, tissu pulmonaire de souris, tissu rénal humain
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

11861-1-AP cible RAMP3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAMP3 Protéine recombinante Ag2438
• Nom complet: receptor (G protein-coupled) activity modifying protein 3
• Masse moléculaire calculée: 16.5 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC022304
• Symbole du gène: RAMP3
• Identification du gène (NCBI): 10268
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The receptor activity-modifying proteins (RAMPs) and the calcitonin receptor-like receptor (CRLR) are both required to generate adrenomedullin (AM) and calcitonin gene-related peptide (CGRP) receptors. During the process, RAMP3 transports the glycosylated 58-kD CGRPR to the cell surface. The formation of CRLR-RAMP heterodimer is essential for the proper cell surface targeting and pharmacological characteristics of both CGRP and AM receptors.