Anticorps Polyclonal de lapin anti-RAI14

• Phare
• Validé par KD/KO

RAI14 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 17507-1-AP

Synonymes

Ankycorbin, KIAA1334, NORPEG, RAI13, RAI14, retinoic acid induced 14

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Galerie de données de validation

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  WB analysis of HeLa using 17507-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17507-1-AP (RAI14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17507-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17507-1-AP (RAI14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17507-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17507-1-AP (RAI14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17507-1-AP

  IP result of anti-RAI14 (IP:17507-1-AP, 5ug; Detection:17507-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IF Staining of HeLa using 17507-1-AP

  Immunofluorescent analysis of Hela cells, using RAI14 antibody 17507-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu placentaire humain, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

17507-1-AP cible RAI14 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAI14 Protéine recombinante Ag11708
• Nom complet: retinoic acid induced 14
• Masse moléculaire calculée: 980 aa, 110 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC052988
• Symbole du gène: RAI14
• Identification du gène (NCBI): 26064
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	WB	Genes Dev; Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein; Authors - Megan L Norris
human,mouse	WB,IP	Elife; Retinoic acid-induced protein 14 controls dendritic spine dynamics associated with depressive-like behaviors.; Authors - Soo Jeong Kim
mouse	WB,IF	Cells; Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model.; Authors - Natalie Längst
human	WB	Cell Transplant; RAI14 Regulated by circNFATC3/miR-23b-3p axis Facilitates Cell Growth and Invasion in Gastric Cancer.; Authors - XinXin Yan
rat	WB,IF,IP	PLoS One; Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*.; Authors - Xiaojing Qian ; KD Validated
mouse	WB,IF	PeerJ; Retinoic Acid Induced Protein 14 (Rai14) is dispensable for mouse spermatogenesis.; Authors - Yangyang Wu ; KO Validated