Anticorps Polyclonal de lapin anti-RAD51AP1

• Phare
• Validé par KD/KO

RAD51AP1 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 11255-1-AP

Synonymes

PIR51, RAD51 associated protein 1, RAD51 interacting protein, RAD51AP1

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Galerie de données de validation

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  WB analysis using 11255-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11255-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11255-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 11255-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11255-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 11255-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11255-1-AP (RAD51AP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Raji, tissu cérébral humain, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

11255-1-AP cible RAD51AP1 dans les applications de WB, IF/ICC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAD51AP1 Protéine recombinante Ag1781
• Nom complet: RAD51 associated protein 1
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC016330
• Symbole du gène: RAD51AP1
• Identification du gène (NCBI): 10635
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon physical interaction of the two HR factors [PMID:17996710,17996711].

Publications

Species	Application	Title
human	WB,IF	Nature; RNA transcripts stimulate homologous recombination by forming DR-loops.; Authors - Jian Ouyang ; KD Validated
human	WB	Mol Cell; TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switch; Authors - Tribhuwan Yadav
human	WB	Oncogene; BRD4 inhibition sensitizes cervical cancer to radiotherapy by attenuating DNA repair.; Authors - Mengdong Ni
human,mouse	IF	J Biol Chem; Pre-rRNA facilitates the recruitment of RAD51AP1 to DNA double-strand breaks; Authors - Linlin Chen
human	WB	Front Genet; Systematic pan-cancer analysis showed that RAD51AP1 was associated with immune microenvironment, tumor stemness, and prognosis; Authors - Renwang Liu
mouse	WB	J Cell Biochem; The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes.; Authors - Shuhei Ishikura