Anticorps Polyclonal de lapin anti-RAD23A

RAD23A Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 11364-1-AP

Synonymes

HHR23A, HR23A, RAD23A

---

Galerie de données de validation

• 

  WB analysis of mouse testis using 11364-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11364-1-AP (RAD23A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11364-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11364-1-AP (RAD23A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

11364-1-AP cible RAD23A dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAD23A Protéine recombinante Ag1907
• Nom complet: RAD23 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC014026
• Symbole du gène: RAD23A
• Identification du gène (NCBI): 5886
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAD23A, also named as HHR23A, belongs to the RAD23 family. It is a multiubiquitin chain receptor involved in modulation of proteasomal degradation. RAD23A binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. It is proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. It is involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC. It is also involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages and is required for the association of HIV-1 vpr with the host proteasome. This antibody is a rabbit polyclonal antibody raised against full length RAD23A of human origin.

Publications

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
human	WB	Cancer Lett; Radioprotective effects of genistein on HL-7702 cells via the inhibition of apoptosis and DNA damage.; Authors - Lihua Song