Anticorps Polyclonal de lapin anti-RAD18

• Phare
• Validé par KD/KO

RAD18 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 18333-1-AP

Synonymes

hHR18, hRAD18, RAD18, RAD18 homolog (S. cerevisiae), RING finger protein 73, RNF73

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Galerie de données de validation

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  WB analysis of K-562 using 18333-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18333-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18333-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18333-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 18333-1-AP

  IP result of anti-RAD18 (IP:18333-1-AP, 3ug; Detection:18333-1-AP 1:300) with K-562 cells lysate 2000ug.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules A549, cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 7 publications below

Informations sur le produit

18333-1-AP cible RAD18 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAD18 Protéine recombinante Ag12944
• Nom complet: RAD18 homolog (S. cerevisiae)
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 75 kDa, 85 kDa
• Numéro d’acquisition GenBank: BC001302
• Symbole du gène: RAD18
• Identification du gène (NCBI): 56852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAD18(E3 ubiquitin-protein ligase RAD18) is lalso named as RNF73, hHR18 and belongs to the RAD18 family. It is involved in postreplication repair of UV-damaged DNA. RAD18 protein is detected in human cells as two major bands at 75 and 85 kDa by Western blot. The bands are identified as nonubiquitinated and monoubiquitinated forms of Rad18, respectively. Monoubiquitinated RAD18 is detected mainly in the cytoplasm, whereas nonubiquitinated RAD18 is detected predominantly in the nuclei(PMID:15509568). This antibody is specific to RAD18.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Regulation of the DNA damage response on male meiotic sex chromosomes.; Authors - Lu Lin-Yu LY
human	WB	Theranostics; A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.; Authors - Qi Zhang
human	IF	Nucleic Acids Res; REV1 and polymerase ζ facilitate homologous recombination repair.; Authors - Sharma Shilpy S
human	WB	J Cell Sci; RNF168 forms a functional complex with RAD6 during the DNA damage response.; Authors - Liu Chao C
human	IF	Cell Cycle; A small ubiquitin binding domain inhibits ubiquitin-dependent protein recruitment to DNA repair foci.; Authors - Corey M Helchowski
human	WB,IF	Mol Pharmacol; DNA polymerase ζ is a major determinant of resistance to platinum-based chemotherapeutic agents.; Authors - Sharma Shilpy S

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alexandra (Verified Customer) (03-20-2024)

The middle part of the picture is irrelevant (other sample, other antibody), but I did not want to modify the picture. The antibody works perfect, on the picture the endogenous Rad18 can be seen. It works fine for the endogenous protein visualisation in 1000x dilution, incubation ON.

• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1000x
• Cell Tissue Type: U2OS human osteosarcoma cell line

RAD18 Antibody Western Blot,Immunohistochemistry,Immunoprecipitation,Cell culture validation (1000x dilution) in U2OS human osteosarcoma cell line (Cat no:18333-1-AP)