Anticorps Polyclonal de lapin anti-RAC2

• Phare
• Validé par KD/KO

RAC2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 10735-1-AP

Synonymes

EN 7, Gx, HSPC022, p21 Rac2, RAC2, Small G protein

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Galerie de données de validation

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  WB analysis using 10735-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10735-1-AP (RAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 10735-1-AP

  IP result of anti-RAC2 (IP:10735-1-AP, 3ug; Detection:10735-1-AP 1:300) with HL-60 cells lysate 4000ug.

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  IHC staining of human tonsillitis using 10735-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10735-1-AP (RAC2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10735-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10735-1-AP (RAC2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10735-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10735-1-AP (RAC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10735-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10735-1-AP (RAC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HL-60, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: cellules HL-60
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below

Informations sur le produit

10735-1-AP cible RAC2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAC2 Protéine recombinante Ag1202
• Nom complet: ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC001485
• Symbole du gène: RAC2
• Identification du gène (NCBI): 5880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome.

Publications

Species	Application	Title
mouse	WB	Nat Genet; In vivo RNAi screening identifies regulators of actin dynamics as key determinants of lymphoma progression.; Authors - Meacham Corbin E CE ; KD Validated
human	WB	J Exp Clin Cancer Res; Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.; Authors - Yu Lian
human	WB	Front Mol Neurosci; Quantitative Proteomic Analysis of Mouse Sciatic Nerve Reveals Post-injury Upregulation of ADP-Dependent Glucokinase Promoting Macrophage Phagocytosis.; Authors - Kai Zhang
human	WB	Oncol Rep; Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer.; Authors - Hao Bo
human	WB	Biochem Biophys Res Commun; Hsa_circ_001659 serves as a novel diagnostic and prognostic biomarker for colorectal cancer.; Authors - Baoyu He
human	WB	Tumour Biol; AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.; Authors - Zhaoyang Zeng