Anticorps Polyclonal de lapin anti-RAB6B

• Phare
• Validé par KD/KO

RAB6B Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 10340-1-AP

Synonymes

RAB6B, Ras related protein Rab 6B

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Galerie de données de validation

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  WB analysis of mouse brain using 10340-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10340-1-AP (RAB6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10340-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10340-1-AP (RAB6B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 10340-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10340-1-AP (RAB6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10340-1-AP

  IP result of anti-RAB6B (IP:10340-1-AP, 3ug; Detection:10340-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 10340-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10340-1-AP (RAB6B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10340-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10340-1-AP (RAB6B antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of C6 using 10340-1-AP

  Immunofluorescent analysis of C6 cells using 10340-1-AP (RAB6B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules C6, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules C6

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

10340-1-AP cible RAB6B dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB6B Protéine recombinante Ag0322
• Nom complet: RAB6B, member RAS oncogene family
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC002510
• Symbole du gène: RAB6B
• Identification du gène (NCBI): 51560
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The human RAB genes share structural and biochemical properties with the Ras gene superfamily. Accumulating data suggests an important role for RAB proteins either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from endoplasmic reticulum to the Golgi complex and to secretory vesicles involves the movement of carrier vesicles, a process that appears to involve RAB protein function. Rab6A has been shown to be a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Rab6B is encoded by an independent gene which is located on chromosome 3 region q21-q23. In contrast to Rab6A whose expression is ubiquitous, Rab6B is expressed in a tissue and cell-type specific manner. Rab6B is predominantly expressed in brain and the neuroblastoma cells. In brain, Rab6B was found to be specifically expressed in microglia, pericytes and Purkinje cells. Endogenous Rab6B localises to the Golgi apparatus and to ERGIC-53-positive vesicles. Comparable studies between Rab6A and Rab6B revealed distinct biochemical and cellular properties. Rab6B displays lower GTP-binding activities and is distributed over Golgi and ER membranes, whereas Rab6A is more restricted to the Golgi apparatus. Since the GTP-bound form of Rab6B does interact with all known Rab6A effectors, including Rabkinesin-6, the results suggest a cell-type specific role for Rab6B in retrograde membrane traffic at the level of the Golgi complex.

Publications

Species	Application	Title
rat	IF	J Neuroimmunol; Autoantibody against the Rab6A/Rab6B in primary autoimmune cerebellar ataxia associated with Sjogren's syndrome: A case report.; Authors - Liyuan Guo
mouse	WB	Proteome Sci; PMID: 29760588; Authors - Elisa Ghelfi
mouse	WB	EMBO Rep; RAB6 and dynein drive post-Golgi apical transport to prevent neuronal progenitor delamination; Authors - Sabine Bardin ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sahil (Verified Customer) (09-02-2020)

Works great as expected.

• Applications: Western Blot