Anticorps Polyclonal de lapin anti-RAB6A

• Phare
• Validé par KD/KO

RAB6A Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 10187-2-AP

Synonymes

Rab 6, RAB6, RAB6A, RAB6A', RAB6B, Ras related protein Rab 6A

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Galerie de données de validation

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  WB analysis using 10187-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10187-2-AP (RAB6A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10187-2-AP

  IP result of anti-RAB6A (IP:10187-2-AP, 4ug; Detection:10187-2-AP 1:1000) with mouse brain tissue lysate 1840 ug.

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  IF Staining of HeLa using 10187-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10187-2-AP (RAB6A antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

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  IF Staining of HeLa using 10187-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB6A antibody (10187-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10187-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB6A antibody (10187-2-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

10187-2-AP cible RAB6A dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB6A Protéine recombinante Ag0240
• Nom complet: RAB6A, member RAS oncogene family
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC003617
• Symbole du gène: RAB6A
• Identification du gène (NCBI): 5870
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Members of the Rab subfamily of small GTPases play an important role in the regulation of intracellular transport routes. Rab6, localized to the Golgi apparatus, has been shown to be a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). The expression of Rab6 is ubiquitous.

Publications

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB	J Clin Invest; A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.; Authors - Xiaochao Tan
rat	IF	J Neuroimmunol; Autoantibody against the Rab6A/Rab6B in primary autoimmune cerebellar ataxia associated with Sjogren's syndrome: A case report.; Authors - Liyuan Guo
mouse	WB	Development; A crucial role for Arf6 in the response of commissural axons to Slit.; Authors - Mariko Kinoshita-Kawada ; KD Validated
human	WB	Nat Commun; SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.; Authors - Seiichi Koike

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (01-25-2019)

Very good, both for WB and IF assays.By WB, it gives a strong specific band at the expected molecular weight; by IF, PFA fixation, it labels Rab6 at the Golgi and the cilia.

• Applications: Western Blot, Immunofluorescence,