Anticorps Polyclonal de lapin anti-RAB4A

RAB4A Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10347-1-AP

Synonymes

HRES 1/RAB4, RAB4, RAB4A, Ras related protein Rab 4A

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Galerie de données de validation

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  WB analysis using 10347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10347-1-AP (RAB4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10347-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10347-1-AP (RAB4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10347-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10347-1-AP (RAB4A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 10347-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10347-1-AP (RAB4A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 10347-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10347-1-AP (RAB4A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10347-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB4A antibody (10347-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules Neuro-2a, cellules PC-12, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cardiaque humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

10347-1-AP cible RAB4A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB4A Protéine recombinante Ag0357
• Nom complet: RAB4A, member RAS oncogene family
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24-28 kDa
• Numéro d’acquisition GenBank: BC002438
• Symbole du gène: RAB4A
• Identification du gène (NCBI): 5867
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The human RAB genes share structural and biochemical properties with the Ras gene superfamily, and RAB4 (synonym: RAB4A) is a small GTPase belonging to this Ras superfamily. Accumulating data suggests an important role for RAB proteins either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from endoplasmic reticulum to the Golgi complex and to secretory vesicles involves the movement of carrier vesicles, a process that appears to involve RAB protein function. RAB4 may also function as regulator along the receptor recycling pathway.

Publications

Species	Application	Title
		Nat Commun; CHML promotes liver cancer metastasis by facilitating Rab14 recycle.; Authors - Tian-Wei Chen
mouse	IF	MBio; Yersinia pestisTargets the Host Endosome Recycling Pathway during the Biogenesis of theYersinia-Containing Vacuole To Avoid Killing by Macrophages.; Authors - Michael G Connor
human	WB	Virology; Characterization of intracellular dynamics of inoculated PrP-res and newly generated PrP(Sc) during early stage prion infection in Neuro2a cells.; Authors - Takeshi Yamasaki
mouse	IF	Pathog Dis; Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages.; Authors - Jia-Hui Deng
mouse	WB	FASEB J; Tsg101 positively regulates physiologic-like cardiac hypertrophy through FIP3-mediated endosomal recycling of IGF-1R.; Authors - Kobina Essandoh