Anticorps Polyclonal de lapin anti-RAB37

RAB37 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 13051-1-AP

Synonymes

RAB37, Ras related protein Rab 37

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Galerie de données de validation

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  WB analysis using 13051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13051-1-AP (RAB37 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13051-1-AP (RAB37 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13051-1-AP

  IP result of anti-RAB37 (IP:13051-1-AP, 4ug; Detection:13051-1-AP 1:3000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of mouse spleen using 13051-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13051-1-AP (RAB37 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 13051-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13051-1-AP (RAB37 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen tissue using 13051-1-AP

  IHC result from Barry Bradford of 13051-1-AP (1:1,000) in Mouse Spleen.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

13051-1-AP cible RAB37 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB37 Protéine recombinante Ag3717
• Nom complet: RAB37, member RAS oncogene family
• Masse moléculaire calculée: 223 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC016615
• Symbole du gène: RAB37
• Identification du gène (NCBI): 326624
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB37, as a small GTPase, plays important roles in several cellular processes through intracellular membrane traffic. RAB37 can regulate insulin secretion in β-cells 7 and TNF-α secretion from activated macrophages. In allergic responses, RAB37 regulated mast cell degranulation. RAB37 has been identified to be a tumor suppressor and it regulated exocytosis of several proteins including TIMP metallopeptidase inhibitor 1 (TIMP1), secreted frizzled-related protein-1 (SFRP1) 12, thrombospondin-1 (TSP1), and soluble ST2.

Publications

Species	Application	Title
human	WB,IHC,IF	Clin Cancer Res; RAB37 Hypermethylation Regulates Metastasis and Resistance to Docetaxel-Based Induction Chemotherapy in Nasopharyngeal Carcinoma.; Authors - Yingqin Li
mouse	WB,IHC,IF,IP	Autophagy; Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo; Authors - Shan-Ying Wu
human	WB	Cell Death Differ; RAB37 interacts directly with ATG5 and promotes autophagosome formation via regulating ATG5-12-16 complex assembly.; Authors - Yue Sheng
human	IHC	Mol Cancer; Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.; Authors - Yu-Chan Chang
canine	WB	Cell Rep; Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.; Authors - Takahide Matsui
human	WB	Cell Rep; RPGR is a guanine nucleotide exchange factor for the small GTPase RAB37 required for retinal function via autophagy regulation; Authors - Ruhong Ying