Anticorps Polyclonal de lapin anti-RAB2B

RAB2B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11756-1-AP

Synonymes

RAB2B, Ras related protein Rab 2B

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Galerie de données de validation

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  WB analysis of human brain using 11756-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11756-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11756-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11756-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11756-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11756-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

11756-1-AP cible RAB2B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB2B Protéine recombinante Ag2352
• Nom complet: RAB2B, member RAS oncogene family
• Masse moléculaire calculée: 216 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC020839
• Symbole du gène: RAB2B
• Identification du gène (NCBI): 84932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize both RAB2A and RAB2B.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion; Authors - Yuxin Sun ; KD Validated
human	IHC	Dis Markers; Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma; Authors - Xiubing Chen
mouse	WB	Dev Cell; Setdb1 protects genome integrity in murine muscle stem cells to allow for regenerative myogenesis and inflammation; Authors - Pauline Garcia