Anticorps Polyclonal de lapin anti-RAB27A-specific

• Phare
• Validé par KD/KO

RAB27A-specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 16868-1-AP

Synonymes

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAB27A-specific, RAM, Ras related protein Rab 27A

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Galerie de données de validation

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  WB analysis using 16868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16868-1-AP (RAB27A-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K562 using 16868-1-AP

  WB result of RAB27A antibody (16868-1-AP, 1:1000) with si-Control and si-RAB27A transfected K562 cells.

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  WB analysis of HL-60 using 16868-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16868-1-AP (RAB27A-specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 16868-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 16868-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 16868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules MG U-87, cellules PC-12
• Résultats positifs en IHC: tissu de lymphome humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

16868-1-AP cible RAB27A-specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: RAB27A, member RAS oncogene family
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: NM_004580
• Symbole du gène: RAB27A
• Identification du gène (NCBI): 5873
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

Publications

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun; Involvement of Rab11 in osteoblastic differentiation: Its up-regulation during the differentiation and by tensile stress; Authors - Lay Thant