Anticorps Polyclonal de lapin anti-Rab23

• Phare
• Validé par KD/KO

Rab23 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 11101-1-AP

Synonymes

HSPC137, RAB23, Ras related protein Rab 23

---

Galerie de données de validation

• 

  WB analysis of mouse brain using 11101-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11101-1-AP (RAB23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11101-1-AP

  WB result of RAB23 antibody (11101-1-AP, 1:3000) with si-Control and si-Rab23 transfected HEK293 cells.

• 

  WB analysis of human brain using 11101-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11101-1-AP (RAB23 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11101-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11101-1-AP (RAB23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11101-1-AP

  IP result of anti-Rab23 (IP:11101-1-AP, 3ug; Detection:11101-1-AP 1:1000) with mouse brain tissue lysate 7000ug.

• 

  IHC staining of human liver cancer using 11101-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11101-1-AP (RAB23 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 11101-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11101-1-AP (RAB23 Antibody) at dilution of 1:200 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Informations sur le produit

11101-1-AP cible Rab23 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Rab23 Protéine recombinante Ag1588
• Nom complet: RAB23, member RAS oncogene family
• Masse moléculaire calculée: 237 aa, 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC015021
• Symbole du gène: RAB23
• Identification du gène (NCBI): 51715
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).

Publications

Species	Application	Title
human,mouse	WB,IF	Curr Biol; Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex.; Authors - Andreas Gerondopoulos ; KD Validated
mouse	WB,IHC	Elife; RAB23 coordinates early osteogenesis by repressing FGF10-pERK1/2 and GLI1.; Authors - Md Rakibul Hasan
human	WB,IF	Oncotarget; Rab23 promotes squamous cell carcinoma cell migration and invasion via integrin β1/Rac1 pathway.; Authors - Qiang Jian ; KD Validated
human	WB	J Cell Sci; Differential role of Rab proteins in ciliary trafficking: Rab23 regulates smoothened levels.; Authors - Boehlke Christopher C ; KD Validated
human	WB,IHC	J Cancer Res Clin Oncol; MicroRNA-367-3p directly targets RAB23 and inhibits proliferation, migration and invasion of bladder cancer cells and increases cisplatin sensitivity; Authors - Xifeng Wei ; KD Validated
human,mouse	WB,IF	Exp Dermatol; Silencing of Rab23 by siRNA inhibits ultraviolet B-induced melanogenesis via downregulation of PKA/CREB/MITF.; Authors - Xuanjin Wei ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Guorong (Verified Customer) (11-22-2022)

Multiple bands distributed at 50 and 75 kDa were observed . Does not represent the performance in other kind of samples

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

Rab23 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:11101-1-AP)