Anticorps Polyclonal de lapin anti-RAB1B

• Phare
• Validé par KD/KO

RAB1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 17824-1-AP

Synonymes

Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2

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Galerie de données de validation

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  WB analysis using 17824-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17824-1-PBS

  HeLa cells were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 17824-1-AP

  HAP1 (WT and RAB1A and RAB1B KO) lysates prepared with RIPA buffer, 30 μg of protein loaded. 17824-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HeLa using 17824-1-AP

  WB result of RAB1B antibody (17824-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB1B transfected HeLa cells.

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  WB analysis of A375 using 17824-1-PBS

  A375 cells were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 17824-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 17824-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 17824-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 17824-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 17824-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17824-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB1B antibody (17824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 17824-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 17824-1-AP (RAB1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules A431, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IHC: tissu cardiaque humain, tissu cardiaque de souris, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17824-1-AP cible RAB1B dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB1B Protéine recombinante Ag12197
• Nom complet: RAB1B, member RAS oncogene family
• Masse moléculaire calculée: 201 aa, 22 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC071169
• Symbole du gène: RAB1B
• Identification du gène (NCBI): 81876
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ras-related protein Rab-1B (RAB1B) belongs to the small GTPase superfamily and Rab family. Rab1b, a GTPase regulating secretory transport,  was recently associated with targeting proteins to LDs in a Drosophila RNAi screen. Rab1b recruits lipid-synthesizing enzymes, facilitating Lipid droplets (LDs) growth. The small GTPase Rab1b interacts with diacylglycerol acyltransferase 2 to facilitate its targeting to the surface of lipid droplets (PMID: 38809969). Rab1b, an extensively studied and established master regulator of ER-to-Golgi transport. The mechanism of Rab1b's function is to promote ER to LD surface targeting of those triglyceride-synthesizing enzymes  that can associate with the unique lipid monolayer comprising the LD surface (PMID: 38809969). Rab1b is a regulatory protein involved in both COPI and COPII transport (PMID: 17429068).

Publications

Species	Application	Title
human	WB	Cancer Cell; Rab1A Is an mTORC1 Activator and a Colorectal Oncogene.; Authors - Janice D Thomas
human	WB	Nat Cell Biol; A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.; Authors - Reiling Jan H JH
human	IF	Dev Cell; SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.; Authors - Rui Yan
human	IHC	Aging (Albany NY); Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer.; Authors - Xian-Zi Yang
rat	WB	Front Pharmacol; Fuyuan Xingnao Decoction Promotes Angiogenesis Through the Rab1/AT1R Pathway in Diabetes Mellitus Complicated With Cerebral Infarction.; Authors - Dong Deng
human	WB	Oncol Rep; Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer.; Authors - Hao Bo