Anticorps Polyclonal de lapin anti-Rab18

• Phare
• Validé par KD/KO

Rab18 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(18)

N° de cat : 11304-1-AP

Synonymes

Ras-related protein Rab-18, Ras related protein Rab 18, RAB18LI1

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Galerie de données de validation

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  WB analysis using 11304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11304-1-AP (Rab18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 11304-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11304-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11304-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11304-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11304-1-AP

  IP result of anti-Rab18 (IP:11304-1-AP, 4ug; Detection:11304-1-AP 1:2000) with mouse testis tissue lysate 1120 ug.

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  IP experiment of rat testis using 11304-1-AP

  IP result of anti-Rab18 (IP:11304-1-AP, 4ug; Detection:11304-1-AP 1:2000) with rat testis tissue lysate 4000 ug.

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  IHC staining of mouse brain using 11304-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11304-1-AP (Rab18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11304-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11304-1-AP (Rab18 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11304-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11304-1-AP (Rab18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11304-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11304-1-AP (Rab18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 11304-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Rab18 antibody (11304-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 11304-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Rab18 antibody (11304-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 11304-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Rab18 antibody (11304-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules PC-3, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral humain, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu testiculaire de souris, tissu testiculaire de rat
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 16 publications below
• IHC: See 7 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Informations sur le produit

11304-1-AP cible Rab18 dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Rab18 Protéine recombinante Ag1841
• Nom complet: RAB18, member RAS oncogene family
• Masse moléculaire calculée: 206 aa, 23 kDa
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC015014
• Symbole du gène: RAB18
• Identification du gène (NCBI): 22931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB18 is a member of a family of Ras-related small GTPases. RAB18 plays a role in apical endocytosis/recycling, and may be implicated in transport between the plasma membrane and early endosomes. It also plays a key role in eye and brain development and neurodegeneration. Defects in RAB18 are associated with Warburg micro syndrome type 3.

Publications

Species	Application	Title
human	WB,IF	J Clin Invest; RINT1 deficiency disrupts lipid metabolism and underlies a complex hereditary spastic paraplegia; Authors - Nathalie Launay
monkey	WB	J Cell Biol; Rab18 and a Rab18 GEF complex are required for normal ER structure.; Authors - Andreas Gerondopoulos
mouse	IF	Dev Cell; KIF3B promotes a PI3K signaling gradient causing changes in a Shh protein gradient and suppressing polydactyly in mice; Authors - Shuo Wang
human	WB	PLoS Pathog; Rab18 Binds to Hepatitis C Virus NS5A and Promotes Interaction between Sites of Viral Replication and Lipid Droplets.; Authors - Salloum Shadi S
mouse	WB	Food Chem; Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity.; Authors - Shanshan Hu
human	WB,IHC	Carcinogenesis; Hepatitis B virus X protein upregulates oncogene Rab18 to result in the dysregulation of lipogenesis and proliferation of hepatoma cells.; Authors - You Xiaona X ; KD Validated

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