Anticorps Polyclonal de lapin anti-RAB11A-Specific

• Phare
• Validé par KD/KO

RAB11A-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 20229-1-AP

Synonymes

RAB11, Rab 11, RAB11A/B, RAB11A, Rab-11

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Galerie de données de validation

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  WB analysis using 20229-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20229-1-AP

  WB result of RAB11A-Specific antibody (20229-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB11A-Specific transfected HeLa cells.

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  WB analysis of rat brain using 20229-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 20229-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20229-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 20229-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20229-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 20229-1-AP

  IP result of anti-RAB11A-Specific (IP:20229-1-AP, 3ug; Detection:20229-1-AP 1:700) with SH-SY5Y cells lysate 3000ug.

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  IHC staining of human small intestine using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human small intestine using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human small intestine using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HEK-293 using 20229-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RAB11A-Specific antibody (20229-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules MCF-7, cellules PC-12, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral humain, tissu de côlon de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en IHC: tissu d'intestin grêle humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 10 publications below

Informations sur le produit

20229-1-AP cible RAB11A-Specific dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: RAB11A, member RAS oncogene family
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: NM_004663
• Symbole du gène: RAB11A
• Identification du gène (NCBI): 8766
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939). The antibody is specific to RAB11A, and has no cross reaction to RAB11B.

Publications

Species	Application	Title
human	IF	J Thromb Haemost; Increased potency of recombinant VWF D'D3 albumin fusion proteins engineered for enhanced affinity for coagulation factor VIII.; Authors - Jenny Chia
human	WB	Proc Natl Acad Sci U S A; ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.; Authors - Humbert Melissa C MC ; KD Validated
		J Cell Biol; BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia.; Authors - William J Monis
mouse	IF	EMBO Rep; Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.; Authors - Ye Chen
mouse	WB	Cell Mol Gastroenterol Hepatol; Rab9 Mediates Pancreatic Autophagy Switch From Canonical to Noncanonical, Aggravating Experimental Pancreatitis.; Authors - Olga A Mareninova
human	IF	Br J Pharmacol; An orally available hypoglycaemic peptide taken up by caveolae transcytosis displays improved hypoglycaemic effects and body weight control in db/db mice.; Authors - Weisheng Lu