Anticorps Polyclonal de lapin anti-RAB11A/B

• Phare
• Validé par KD/KO

RAB11A/B Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (4)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

34

Avis (2)

N° de cat : 15903-1-AP

Synonymes

Rab 11, RAB11, RAB11A, RAB11A/B, Ras related protein Rab 11A, YL8

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Galerie de données de validation

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  WB analysis of mouse spleen using 15903-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15903-1-AP (RAB11A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15903-1-AP

  WB result of RAB11A/B antibody (15903-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB11A/B transfected HeLa cells.

• 

  WB analysis of Jurkat using 15903-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15903-1-AP (RAB11A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 15903-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15903-1-AP (RAB11A/Bantibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15903-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15903-1-AP (RAB11A/B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 15903-1-AP

  IP result of anti-RAB11A/B (IP:15903-1-AP, 4ug; Detection:15903-1-AP 1:1000) with mouse spleen tissue lysate 1920 ug.

• 

  IP experiment of mouse spleen using 15903-1-AP

  IP result of anti-RAB11A/B (IP:15903-1-AP, 3ug; Detection:15903-1-AP 1:500) with mouse spleen tissue lysate 4000ug.

• 

  IP experiment of Jurkat using 15903-1-AP

  IP result of anti-RAB11A/B (IP:15903-1-AP, 4ug; Detection:15903-1-AP 1:3000) with Jurkat cells lysate 2320 ug.

• 

  IHC staining of human heart using 15903-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15903-1-AP (RAB11A/B antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 15903-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15903-1-AP (RAB11A/B antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 15903-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11A/B antibody (15903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 15903-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11A/B antibody (15903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of U2OS using 15903-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using RAB11A/B antibody (15903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 15903-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 15903-1-AP (RAB11A/B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15903-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RAB11A/B antibody (15903-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules HeLa, cellules Jurkat, cellules MCF-7, cellules SH-SY5Y
• Résultats positifs en IP: cellules Jurkat, tissu splénique de souris
• Résultats positifs en IHC: tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 6 publications below
• IF: See 19 publications below
• CoIP: See 3 publications below

Informations sur le produit

15903-1-AP cible RAB11A/B dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: bovin, canin, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB11A/B Protéine recombinante Ag8742
• Nom complet: RAB11A, member RAS oncogene family
• Masse moléculaire calculée: 216 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC013348
• Symbole du gène: RAB11A
• Identification du gène (NCBI): 8766
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB11 has two isoform genes RAB11A(YL8) and RAB11B(YPT3), and belongs to the small GTPase superfamily and Rab family. It regulates endocytic recycling. RAB11 may exert its functions by interacting with multiple effector proteins in different complexes. It acts as a major regulator of membrane delivery during cytokinesis. RAB11 regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. This antibody can recognize both RAB11A(24 kDa) and RAB11B(24 kDa).

Publications

Species	Application	Title
	IF	Nanomicro Lett; Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling; Authors - Junji Ren
human	IF	Nat Cell Biol; miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.; Authors - Cao Jingli J
canine	IF	J Cell Biol; Septins promote macropinosome maturation and traffic to the lysosome by facilitating membrane fusion.; Authors - Lee Dolat
human	WB,CoIP	Cell Mol Life Sci; KIF13B mediates VEGFR2 recycling to modulate vascular permeability; Authors - Hyun-Dong Cho
human	IF	Oncogene; Piccolo mediates EGFR signaling and acts as a prognostic biomarker in esophageal squamous cell carcinoma.; Authors - W Zhang
human	WB,IF,CoIP	Clin Transl Med; ANXA6 suppresses the tumorigenesis of cervical cancer through autophagy induction.; Authors - Xin Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (06-16-2021)

Immunocytochemistry labelling of (4% PFA) fixed SH-SY5Y cell line that was pre-treated by the drug of interest and labelled by RAB11 polyclonal antibody at dilution of 1:100 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: SH-SY5Y cell line

FH

Stephen (Verified Customer) (09-12-2019)

Oc-2 cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours rab11 antibody incubated 1:300 overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) and Ph647 (red) to visualize DNA and F-actin respectively.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: mouse OC-2 cells

RAB11A/B Antibody Immunofluorescence, validation (1:300 dilution) in mouse OC-2 cells (Cat no:15903-1-AP)