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Anticorps Recombinant de lapin anti-Phospho-mTOR (Ser2448)

Phospho-mTOR (Ser2448) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

3L18

N° de cat : CL555-80596

Synonymes

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2448), RAFT1, Rapamycin target protein 1, RAPT1

Galerie de données de validation

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated Phospho-mTOR (Ser2448) Rec (CL555-80596)

    FC experiment of HeLa using CL555-80596

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite®555 Anti-Human Phospho-mTOR (Ser2448) (CL555-80596, Clone:3L18) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 80% MeOH.

Applications testées

Résultats positifs en cytométriecellules HeLa traitées à la calyculine A, cellules HeLa

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-80596 cible Phospho-mTOR (Ser2448) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, rat

Réactivité Humain, rat
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet FK506 binding protein 12-rapamycin associated protein 1
Masse moléculaire calculée 289 kDa
Poids moléculaire observé250-289 kDa
Numéro d’acquisition GenBankBC117166
Symbole du gène FRAP1
Identification du gène (NCBI) 2475
Conjugaison CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.