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Anticorps Recombinant de lapin anti-Phospho-PDH E1 Alpha (Ser232)

Phospho-PDH E1 Alpha (Ser232) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1L22

N° de cat : CL488-81491

Synonymes

p PDH E1 Alpha, p PDH E1 Alpha (Ser232), p PDH E1 Alpha Ser232, PDH E1 Alpha (Ser232), Phospho PDH E1 Alpha

Galerie de données de validation

Filter:
  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated Phospho-PDH E1 Alpha (CL488-81491)

    FC experiment of HEK-293 using CL488-81491

    1x10^6 HEK-293 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Coralite® Plus 488 Phospho-PDH E1 Alpha (Ser232) Recombinant Antibody (CL488-81491, Clone:1L22)(red), or 0.13 ug CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en FC (Intra)λ phosphatase treated HEK-293 cells,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-81491 cible Phospho-PDH E1 Alpha (Ser232) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet pyruvate dehydrogenase (lipoamide) alpha 1
Masse moléculaire calculée 43 kDa
Poids moléculaire observé 40 kDa
Numéro d’acquisition GenBankBC002406
Symbole du gène PDHA1
Identification du gène (NCBI) 5160
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)

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