Anticorps Recombinant de lapin anti-Phospho-NF-κB p65 (Ser468)

Phospho-NF-κB p65 (Ser468) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

18

CloneNo.

6N1

N° de cat : 82335-1-RR

Synonymes

NFKB3, p65, Phospho-NF-κB p65 (Ser468), RELA, Transcription factor p65

---

Galerie de données de validation

• 

  WB analysis using 82335-1-RR

  Non-treated and Calyculin A treated various cells were subjected to SDS PAGE followed by western blot with 82335-1-RR (Phospho-NF-κB p65 (Ser468) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin antibody (66031-1-Ig) and NF-κB p65 antibody (80979-1-RR) subsequently.

---

Applications testées

• Résultats positifs en WB: cellules HeLa traitées à la calyculine A, cellules NIH/3T3 traitées à la calyculine A

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 18 publications below

Informations sur le produit

82335-1-RR cible Phospho-NF-κB p65 (Ser468) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC011603
• Symbole du gène: RELA
• Identification du gène (NCBI): 5970
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.

Publications

Species	Application	Title
	WB	Redox Biol; Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice; Authors - Boying Zhao
pig	WB	Int J Biol Macromol; Angelica sinensis aboveground part polysaccharide and its metabolite 5-MT ameliorate colitis via modulating gut microbiota and TLR4/MyD88/NF-κB pathway; Authors - Yuan-Feng Zou
human	WB	PLoS Pathog; Negative regulation of type I interferon signaling by integrin-linked kinase permits dengue virus replication; Authors - Yi-Sheng Kao
human	WB	Int Immunopharmacol; Bomidin attenuates inflammation of periodontal ligament stem cells and periodontitis in mice via inhibiting ferroptosis; Authors - Wei Wu
human,rat	WB	Acta Biochim Biophys Sin (Shanghai); Mesenchymal stem cell exosomes inhibit nucleus pulposus cell apoptosis via the miR-125b-5p/TRAF6/NF-κB pathway axis; Authors - Yang Duan
rat	WB	Brain Behav; L-F001, a multifunctional fasudil-lipoic acid dimer, antagonizes hypoxic-ischemic brain damage by inhibiting the TLR4/MyD88 signaling pathway; Authors - Ruiyu Zhou