Anticorps Polyclonal de lapin anti-Phospho-HSP27 (Ser78)

Phospho-HSP27 (Ser78) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 28900-1-AP

Synonymes

DMPK binding protein, heat shock 27kDa protein 2, Heat shock protein beta 2, Hs.78846, Hsp B1, HSP27, Phospho-HSP27 (Ser78)

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Galerie de données de validation

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  WB analysis using 28900-1-AP

  Non-treated HeLa and TNF alpha (HZ-1014) treated HeLa cells were subjected to SDS PAGE followed by western blot with 28900-1-AP (Phospho-HSP27 (Ser78) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 28900-1-AP

  Non-treated and Calyculin A treated HeLa cells and PC-3 cells were subjected to SDS PAGE followed by western blot with 28900-1-AP (Phospho-HSP27 (Ser78) antibody) at dilution of 1:3000 incubated at 4℃ overnight.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées au TNF alpha, cellules HeLa traitées à la calyculine A, cellules PC-3 traitées à la calyculine A

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

28900-1-AP cible Phospho-HSP27 (Ser78) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: heat shock 27kDa protein 1
• Masse moléculaire calculée: 19-23 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC012768
• Symbole du gène: HSPB1
• Identification du gène (NCBI): 3315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)

Publications

Species	Application	Title
human	WB	JOR Spine; Phosphorylated heat shock protein 27 improves the bone formation ability of osteoblasts and bone marrow stem cells from patients with adolescent idiopathic scoliosis; Authors - Sihan He