Anticorps Polyclonal de lapin anti-PYGM-Specific

• Phare
• Validé par KD/KO

PYGM-Specific Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 19716-1-AP

Synonymes

Myophosphorylase, PYGM

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Galerie de données de validation

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  WB analysis of HepG2 using 19716-1-AP

  WB result of PYGM-Specific antibody (19716-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGM-Specific transfected HepG2 cells.

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  WB analysis using 19716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19716-1-AP (PYGM-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 19716-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 19716-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 19716-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 19716-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 19716-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cœur de rat
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below

Informations sur le produit

19716-1-AP cible PYGM-Specific dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: phosphorylase, glycogen, muscle
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 97-105 kDa
• Numéro d’acquisition GenBank: NM_005609
• Symbole du gène: PYGM
• Identification du gène (NCBI): 5837
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PYGM belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) which also known as McArdle disease. The antibody is specific to PYGM.

Publications

Species	Application	Title
mouse	WB	Nature; Glycogen metabolism links glucose homeostasis to thermogenesis in adipocytes.; Authors - Omer Keinan
human	WB	Int J Biol Sci; PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer; Authors - Qian Ji
mouse	WB	Dis Model Mech; Absence of p.R50X Pygm read-through in McArdle disease cellular models.; Authors - Guillermo Tarrasó
mouse	WB	Cells; Proteomic Landscape and Deduced Functions of the Cardiac 14-3-3 Protein Interactome; Authors - Jia-Hua Qu
human	WB	Oncol Lett; Analysis of the expression, function and signaling of glycogen phosphorylase isoforms in hepatocellular carcinoma.; Authors - Lingyu Jiang
human	WB	Clin Proteomics; The different expression of glycogen phosphorylases in renal clear cell renal carcinoma and chromophobe renal carcinoma; Authors - Yang Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Patricia (Verified Customer) (02-25-2020)

Some non-specific bands appeared. Blocked in 5% skim milk and diluted antibody in TBS.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovarian cancer