Anticorps Polyclonal de lapin anti-PYCR2

• Phare
• Validé par KD/KO

PYCR2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (4)

N° de cat : 17146-1-AP

Synonymes

P5C reductase 2, P5CR 2, P5CR2, PYCR2

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Galerie de données de validation

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  WB analysis using 17146-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17146-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 17146-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17146-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17146-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 17146-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR2 antibody (17146-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules L02
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:1500-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

17146-1-AP cible PYCR2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, Singe vert d'Afrique
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PYCR2 Protéine recombinante Ag10918
• Nom complet: pyrroline-5-carboxylate reductase family, member 2
• Masse moléculaire calculée: 320 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC020553
• Symbole du gène: PYCR2
• Identification du gène (NCBI): 29920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.

Publications

Species	Application	Title
human	IF	Cell; Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma.; Authors - Qiang Gao
human	WB	Sci Adv; Loss of attachment promotes proline accumulation and excretion in cancer cells; Authors - Steven E Pilley
mouse	WB	EMBO J; Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.; Authors - Simon Schwörer
human	WB	EMBO J; Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis.; Authors - Zhen Chen
human	WB	Cell Rep; Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.; Authors - Kate E R Hollinshead ; KD Validated
African green monkey	WB	Virol Sin; African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2.; Authors - Sheng Chen ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

balawant (Verified Customer) (12-25-2023)

I have used this antibody . This antibody is working great.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 for western blot and 1:700 for IHC
• Cell Tissue Type: colon tissue of mice and human

FH

Balawant (Verified Customer) (08-06-2022)

This antibody is working great. I have used this antibody for tissue lysate and cell lysate. it has no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon tissue and colon cancer cells

FH

Susmita (Verified Customer) (03-11-2022)

Excellent antibody

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 2000
• Cell Tissue Type: CRC cell line

FH

Iram (Verified Customer) (07-03-2020)

Giving a very sharp and clean result

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human