Anticorps Polyclonal de lapin anti-PYCR1-specific

PYCR1-specific Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 20962-1-AP

Synonymes

P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1

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Galerie de données de validation

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  WB analysis of Raji using 20962-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 20962-1-AP (PYCR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 20962-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 20962-1-AP (PYCR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20962-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20962-1-AP (PYCR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 20962-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 20962-1-AP (PYCR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 20962-1-AP

  IP result of anti-PYCR1-specific (IP:20962-1-AP, 4ug; Detection:20962-1-AP 1:500) with Raji cells lysate 1440ug.

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  IHC staining of human colon cancer using 20962-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20962-1-AP (PYCR1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 20962-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20962-1-AP (PYCR1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Raji, tissu cérébral humain
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

20962-1-AP cible PYCR1-specific dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: pyrroline-5-carboxylate reductase 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: NM_006907
• Symbole du gène: PYCR1
• Identification du gène (NCBI): 5831
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd. This antibody is specific to PYCR1.

Publications

Species	Application	Title
mouse	WB	EMBO J; Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.; Authors - Simon Schwörer
human,mouse	WB	EMBO Mol Med; A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy; Authors - Nneka Southwell