Anticorps Polyclonal de lapin anti-Securin

• Phare
• Validé par KD/KO

Securin Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 18040-1-AP

Synonymes

EAP1, Esp1 associated protein, HPTTG, pituitary tumor transforming 1, PTTG, PTTG1, Securin, Securin / PTTG1, Tumor transforming protein 1, TUTR1

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Galerie de données de validation

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  WB analysis using 18040-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18040-1-AP (Securin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 18040-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 18040-1-AP (Securin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18040-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Securin / PTTG1 antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 18040-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Securin antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules HepG2, cellules MDA-MB-231
• Résultats positifs en IHC: tissu de carcinome urothélial humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

18040-1-AP cible Securin dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Securin Protéine recombinante Ag12670
• Nom complet: pituitary tumor-transforming 1
• Masse moléculaire calculée: 202 aa, 22 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC101834
• Symbole du gène: Securin
• Identification du gène (NCBI): 9232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.

Publications

Species	Application	Title
mouse	IHC,IF	Cell Mol Life Sci; Microglial activation in an amyotrophic lateral sclerosis-like model caused by Ranbp2 loss and nucleocytoplasmic transport impairment in retinal ganglion neurons.; Authors - Kyoung-In Cho
human	WB	Sci Rep; APC/C(CDC20) and APC/C play pivotal roles in the process of embryonic development in Artemia sinica.; Authors - Mengchen Zhang
mouse	WB,IF	Cell Cycle; Chronic restraint stress disturbs meiotic resumption through APC/C-mediated cyclin B1 excessive degradation in mouse oocytes.; Authors - Junyan Sun
mouse	IF	Cryobiology; Melatonin promotes in vitro maturation of vitrified-warmed mouse GV oocytes potentially by modulating MAD2 protein expression of SAC component through MTRs.; Authors - Jinyu Yang
human	WB	Open Med (Wars); The Pseudogene PTTG3P Promotes Cell Migration and Invasion in Esophageal Squamous Cell Carcinoma.; Authors - Zhenhua Zhang ; KD Validated
human	WB,IHC,IF,IP	Oncogene; Phosphorylated PTTG1 switches its subcellular distribution and promotes β-catenin stabilization and subsequent transcription activity; Authors - Xuewen Zhang ; KD Validated