Anticorps Polyclonal de lapin anti-PTRF

• Phare
• Validé par KD/KO

PTRF Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

31

Avis (2)

N° de cat : 18892-1-AP

Synonymes

Cavin 1, FKSG13, PTRF

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Galerie de données de validation

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  WB analysis using 18892-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18892-1-AP (PTRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 18892-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18892-1-AP (PTRF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HT-1080 using 18892-1-AP

  IP result of anti-PTRF (IP:18892-1-AP, 4ug; Detection:18892-1-AP 1:2000) with HT-1080 cells lysate 3600 ug.

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  IHC staining of human pancreas using 18892-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18892-1-AP (PTRF antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brown adipose using 18892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 18892-1-AP (PTRF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 18892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 18892-1-AP (PTRF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 18892-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18892-1-AP (PTRF antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 18892-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTRF antibody (18892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, tissu cardiaque de rat, tissu cardiaque de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules HT-1080,
• Résultats positifs en IHC: tissu pancréatique humain, tissu adipeux brun de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 25 publications below
• IHC: See 6 publications below
• IF: See 8 publications below
• IP: See 2 publications below

Informations sur le produit

18892-1-AP cible PTRF dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTRF Protéine recombinante Ag13486
• Nom complet: polymerase I and transcript release factor
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC008849
• Symbole du gène: PTRF
• Identification du gène (NCBI): 284119
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Polymerase I and transcript release factor(PTRF) is an essential factor in the biogenesis of caveolae, which involve in numerous process, such as signal transduction and membrane and lipid trafficking. PTRF can terminate transciption of RNA Pol I, which involves pausing of transcription by TTF1, and dissocaition of the transcription complex, release the complex from the template. The calculated molecular weight of PTRF is 43 kDa, but modified PTRF is about 50-55kDa. (PMID: 11139612 )

Publications

Species	Application	Title
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
human	WB,IF	Sci Adv; Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.; Authors - Virginia Andrade ; KO Validated
human	WB,IF,IP	Dev Cell; Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2; Authors - Yeping Wu
human	WB,IHC	Autophagy; CAV1-CAVIN1-LC3B-mediated autophagy regulates high glucose-stimulated LDL transcytosis.; Authors - Xiangli Bai ; KD Validated
mouse	WB	Proc Natl Acad Sci U S A; Inducible Fgf13 ablation enhances caveolae-mediated cardioprotection during cardiac pressure overload.; Authors - Eric Q Wei
mouse	IF	Theranostics; PTRF/Cavin-1 enhances chemo-resistance and promotes temozolomide efflux through extracellular vesicles in glioblastoma.; Authors - Eryan Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Celine (Verified Customer) (06-22-2023)

Works very well in Western Blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Melanoma cell lines

PTRF Antibody Western Blot validation (1:1000 dilution) in Melanoma cell lines (Cat no:18892-1-AP)

FH

Maria (Verified Customer) (01-27-2022)

Great antibody. It worked perfectly for IF 1:200 in several cell types (RPE-1, adipocytes, mesenchymal, HeLa or MOVAS), for WB (1:2000) and for IP.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: RPE-1 /MSC/MOVAS