Anticorps Polyclonal de lapin anti-PTPN6

• Phare
• Validé par KD/KO

PTPN6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 24546-1-AP

Synonymes

SHP1, Protein-tyrosine phosphatase 1C, HPTP1C, Hematopoietic cell protein-tyrosine phosphatase, HCPH

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Galerie de données de validation

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  WB analysis using 24546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24546-1-AP (PTPN6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 24546-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 24546-1-AP (SHP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24546-1-AP (SHP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 24546-1-AP

  IP result of anti-PTPN6 (IP:24546-1-AP, 4ug; Detection:24546-1-AP 1:600) with Jurkat cells lysate 3600ug.

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  IHC staining of human colon cancer using 24546-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24546-1-AP (PTPN6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 24546-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PTPN6 antibody (24546-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 24546-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PTPN6 antibody (24546-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 24546-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PTPN6 (24546-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HuH-7, cellules Jurkat
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,
• Résultats positifs en FC (Intra): cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

24546-1-AP cible PTPN6 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTPN6 Protéine recombinante Ag21415
• Nom complet: protein tyrosine phosphatase, non-receptor type 6
• Masse moléculaire calculée: 597 aa, 68 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC002523
• Symbole du gène: PTPN6
• Identification du gène (NCBI): 5777
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTPN6(Tyrosine-protein phosphatase non-receptor type 6) is also named as HCP, PTP1C and belongs to the protein-tyrosine phosphatase family. It regulates muscle INS action in a cell-autonomous manner, further suggesting that the PTPase negatively modulates INS action through down-regulation of both INS signaling to AKT1 and SLC2A4 translocation, as well as SLC2A4 expression(PMID:21952243). It has 4 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IF	Elife; Molecular features underlying differential SHP1/SHP2 binding of immune checkpoint receptors.; Authors - Xiaozheng Xu
human	WB	Oncotarget; Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.; Authors - Ighodaro Igbe
human,mouse	WB	Front Immunol; Host MKRN1-Mediated Mycobacterial PPE Protein Ubiquitination Suppresses Innate Immune Response.; Authors - Yafeng Dou ; KD Validated
human	WB	Int J Biol Sci; PMID: 29805309; Authors - Linna Xie
human	WB	Oxid Med Cell Longev; Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.; Authors - Amara Maryam
human	WB	J Biol Chem; A thiazole-derived oridonin analog exhibits antitumor activity by directly and allosterically inhibiting STAT3.; Authors - Xiaofei Shen