Anticorps Polyclonal de lapin anti-PTPN23

• Phare
• Validé par KD/KO

PTPN23 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 10472-1-AP

Synonymes

HD PTP, HDPTP, KIAA1471, PTP TD14, PTPN23

---

Galerie de données de validation

• 

  WB analysis of A375 using 10472-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10472-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 10472-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10472-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10472-1-AP

  IP result of anti-PTPN23 (IP:10472-1-AP, 5ug; Detection:10472-1-AP 1:500) with HeLa cells lysate 1000ug.

• 

  IHC staining of human colon cancer using 10472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10472-1-AP (PTPN23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 10472-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PTPN23 antibody (10472-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules A375, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Informations sur le produit

10472-1-AP cible PTPN23 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTPN23 Protéine recombinante Ag0765
• Nom complet: protein tyrosine phosphatase, non-receptor type 23
• Masse moléculaire calculée: 179 kDa
• Poids moléculaire observé: 179 kDa
• Numéro d’acquisition GenBank: BC004881
• Symbole du gène: PTPN23
• Identification du gène (NCBI): 25930
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTPN23(Tyrosine-protein phosphatase non-receptor type 23) is also named as HD-PTP,PTP-TD14 and KIAA1471.It encodes a 185-kDa protein which is expressed in human endothelial fromthe umbilical cord and in human Kaposi-spindle cells(PMID:16408268).PTPN23 is a non-transmembrane protein tyrosine phosphatase which contributes to the signal transduction pathways involved in the regulation of cell migration and invasion(PMID:22510412).

Publications

Species	Application	Title
human	IF	Nat Commun; Concerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation.; Authors - Eva Maria Wenzel
human	WB,IHC	Genes Dev; Suppression of protein tyrosine phosphatase N23 predisposes to breast tumorigenesis via activation of FYN kinase.; Authors - Siwei Zhang ; KD Validated
human	IF	EMBO J; ESCRT-mediated lysosome repair precedes lysophagy and promotes cell survival.; Authors - Maja Radulovic
human	WB, IF	Curr Biol; UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.; Authors - Stefani Flavia F
	WB	Curr Biol; Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB.; Authors - Nazim Ali ; KD Validated
human	WB	Cell Rep; Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9.; Authors - Jonathan M Goodwin