Anticorps Polyclonal de lapin anti-PTPMT1

• Phare
• Validé par KD/KO

PTPMT1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 11493-1-AP

Synonymes

DUSP23, MOSP, PLIP, PNAS 129, PTEN like phosphatase, PTPMT1

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Galerie de données de validation

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  WB analysis of mouse pancreas using 11493-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11493-1-AP (PTPMT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat pancreas using 11493-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11493-1-AP (PTPMT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11493-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11493-1-AP (PTPMT1 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris, tissu pancréatique de rat
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11493-1-AP cible PTPMT1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTPMT1 Protéine recombinante Ag2063
• Nom complet: protein tyrosine phosphatase, mitochondrial 1
• Masse moléculaire calculée: 201 aa, 23 kDa
• Poids moléculaire observé: 21-23 kDa
• Numéro d’acquisition GenBank: BC020242
• Symbole du gène: PTPMT1
• Identification du gène (NCBI): 114971
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein Tyrosine Phosphatase localized to the Mitochondrial 1 (PTPMT1) is a highly conserved, dual-specific phosphatase that resides on the matrix-facing side of the inner mitochondrial membrane (IMM). PTPMT1 is a mitochondrial phosphatase and a vital regulator of mitochondrial activities. Current work points to roles for PTPMT1 in the regulation of mitochondrial respiration, dynamics, and ultrastructure through the modulation of pyruvate importation, the tuning of TCA cycle activity, and the synthesis of cardiolipin. PTPMT1 is highly expressed in pancreatic b cells, whose mitochondria have the important function of coupling glucose metabolism to the secretion of insulin. (PMID: 16337125, PMID: 16061174, PMID: 33335799)

Publications

Species	Application	Title
human	WB	Cell Death Dis; 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer; Authors - Yanan Wang
mouse	WB,IF	Mol Cell Biol; A critical role of mitochondrial phosphatase Ptpmt1 in embryogenesis reveals a mitochondrial metabolic stress-induced differentiation checkpoint in embryonic stem cells.; Authors - Shen Jinhua J
human	WB,IHC	Cell Rep; Genome-wide CRISPR-Cas9 knockout library screening identified PTPMT1 in cardiolipin synthesis is crucial to survival in hypoxia in liver cancer.; Authors - Macus Hao-Ran Bao ; KO Validated
human,mouse	WB	Am J Physiol Cell Physiol; PTPMT1 protect cardiomyocytes from necroptosis induced by γ-ray irradiation through alleviate mitochondria injury; Authors - Jing Yi
human	WB	Adv Sci (Weinh); Phosphorylated SHMT2 Regulates Oncogenesis Through m6 A Modification in Lung Adenocarcinoma; Authors - Tianyu Han

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody detects PTPMT1 in mouse heart samples.

• Applications: Western Blot
• Cell Tissue Type: Heart