Anticorps Polyclonal de lapin anti-COX-1/Cyclooxygenase-1

• Phare
• Validé par KD/KO

COX-1/Cyclooxygenase-1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (1)

N° de cat : 13393-1-AP

Synonymes

COX1, COX-1, COX-1/Cyclooxygenase-1, COX3, Cyclooxygenase 1, PCOX1, PGG/HS, PGH synthase 1, PGHS 1, PGHS1, PHS 1, PHS1, Prostaglandin G/H synthase 1, Prostaglandin H2 synthase 1, PTGHS, PTGS1

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Galerie de données de validation

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  WB analysis of HeLa using 13393-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 13393-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 13393-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13393-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13393-1-AP

  IP result of anti-COX-1/Cyclooxygenase-1(IP:13393-1-AP, 4ug; Detection:13393-1-AP 1:500) with HeLa cells lysate 1280 ug.

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  IHC staining of human stomach using 13393-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13393-1-AP (COX-1/Cyclooxygenase-1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 13393-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13393-1-AP (COX-1/Cyclooxygenase-1 antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellule C2C12, cellules Neuro-2a
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13393-1-AP cible COX-1/Cyclooxygenase-1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX-1/Cyclooxygenase-1 Protéine recombinante Ag4055
• Nom complet: prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
• Masse moléculaire calculée: 599 aa, 69 kDa
• Poids moléculaire observé: 60-72 kDa
• Numéro d’acquisition GenBank: BC029840
• Symbole du gène: PTGS1
• Identification du gène (NCBI): 5742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.

Publications

Species	Application	Title
rat	WB	Acta Pharm Sin B; Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage.; Authors - Dominik Bakalarz
	WB	Small; M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation; Authors - Jie Sun
mouse	WB	Theranostics; Nanozyme-mediated catalytic nanotherapy for inflammatory bowel disease.; Authors - Jiulong Zhao
human	WB	Free Radic Biol Med; PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis; Authors - Qiong Sun
human	WB	Acta Biomater; Localized delivery of miRNAs targets cyclooxygenases and reduces flexor tendon adhesions.; Authors - You Lang Zhou
rat	WB	Int J Mol Sci; Arachidonic Acid Metabolites of CYP450 Enzymes and HIF-1α Modulate Endothelium-Dependent Vasorelaxation in Sprague-Dawley Rats under Acute and Intermittent Hyperbaric Oxygenation.; Authors - Zrinka Mihaljević

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Thomas (Verified Customer) (09-18-2020)

HeLa cells were fixed in 4% PFA for 15 mins and permeabilised in 0.1% Triton-X 100 in PBS. Cells were blocked in 1% BSA. Primary antibody solution was diluted at 1:200 in blocking solution and incubated for 1 hour. Goat anti-rabbit 488 Alexa Fluor secondary antibody (1:250 - green) and DAPI (1:2000 - blue) were incubated with cells for 1 hour.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HeLa

COX-1/Cyclooxygenase-1 Antibody Immunofluorescence validation (1:200 dilution) in HeLa (Cat no:13393-1-AP)