Anticorps Polyclonal de lapin anti-PSME2

PSME2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12937-2-AP

Synonymes

PA28B, PA28beta, PSME2, REG beta, REGbeta

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 12937-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12937-2-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12937-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12937-2-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 12937-2-AP

  IP result of anti-PSME2 (IP:12937-2-AP, 4ug; Detection:12937-2-AP 1:500) with MCF-7 cells lysate 2000ug.

• 

  IHC staining of human kidney using 12937-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12937-2-AP (PSME2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12937-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12937-2-AP (PSME2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 12937-2-AP

  Immunofluorescent analysis of MCF-7 cells using 12937-2-AP (PSME2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules MCF-7, tissu cérébral humain, tissu pancréatique de souris, tissu rénal humain
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12937-2-AP cible PSME2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSME2 Protéine recombinante Ag3986
• Nom complet: proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
• Masse moléculaire calculée: 239 aa, 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC019885
• Symbole du gène: PSME2
• Identification du gène (NCBI): 5721
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

Publications

Species	Application	Title
	IF	PLoS Biol; Non-muscle myosins control radial glial basal endfeet to mediate interneuron organization; Authors - Brooke R D'Arcy
human	IHC	Front Genet; PSME2 identifies immune-hot tumors in breast cancer and associates with well therapeutic response to immunotherapy; Authors - Cen Wu
human,mouse	WB	Pharmacol Res; PD-L1 upregulation promotes drug-induced pulmonary fibrosis by inhibiting vimentin degradation; Authors - Qing Li