Anticorps Polyclonal de lapin anti-PSMD3

PSMD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12054-1-AP

Synonymes

P58, Proteasome subunit p58, PSMD3, RPN3, S3

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Galerie de données de validation

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  WB analysis using 12054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12054-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12054-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12054-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12054-1-AP

  IP result of anti-PSMD3 (IP:12054-1-AP, 4ug; Detection:12054-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human colon cancer using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human small intestine using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of human breast cancer using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, tissu cérébral de souris, tissu cérébral humain, tissu rénal de rat, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

12054-1-AP cible PSMD3 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMD3 Protéine recombinante Ag2684
• Nom complet: proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
• Masse moléculaire calculée: 534 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC012302
• Symbole du gène: PSMD3
• Identification du gène (NCBI): 5709
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812).

Publications

Species	Application	Title
human	WB	Sci Rep; Insights into the ubiquitin-proteasome system of human embryonic stem cells.; Authors - Isabel Saez
human	WB	Protein Cell; DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells.; Authors - Yinghao Zhang
human	WB	Cell Death Dis; ALG-2 couples T cell activation and apoptosis by regulating proteasome activity and influencing MCL1 stability.; Authors - Tian-Sheng He
human	WB,IF	Cell Mol Life Sci; The contribution of proteasomal impairment to autophagy activation by C9orf72 poly-GA aggregates; Authors - Mei Pu