Anticorps Polyclonal de lapin anti-PSMD14/POH1

• Phare
• Validé par KD/KO

PSMD14/POH1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 12059-1-AP

Synonymes

PAD1, POH1, PSMD14, PSMD14/POH1, rpn11

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Galerie de données de validation

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  WB analysis of A431 using 12059-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 12059-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12059-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12059-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A375 using 12059-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using PSMD14/POH1 antibody (12059-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A375 using 12059-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using PSMD14/POH1 antibody (12059-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules K-562, tissu cardiaque de rat
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A375,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

12059-1-AP cible PSMD14/POH1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMD14/POH1 Protéine recombinante Ag2694
• Nom complet: proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC009524
• Symbole du gène: PSMD14
• Identification du gène (NCBI): 10213
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The PSMD14 (POH1, also known as Rpn11/MPR1/S13/CepP1) protein is a metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. PSMD14 is highly expressed in the heart and skeletal muscle. In carcinoma cell lines. down-regulation of PSMD14 by siRNA transfection had a considerable impact on cell viability causing cell arrest in the G0-G1 phase, ultimately leading to senescence.

Publications

Species	Application	Title
human	WB,IF	Sci Transl Med; Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa.; Authors - Angel S Byrd
human	WB, IHC, IF	Nat Commun; POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.; Authors - Boshi Wang ; KD Validated
human	WB	Theranostics; Blockade of deubiquitinating enzyme PSMD14 overcomes chemoresistance in head and neck squamous cell carcinoma by antagonizing E2F1/Akt/SOX2-mediated stemness.; Authors - Chao Jing ; KD Validated
human	WB, IF	Theranostics; The PSMD14 inhibitor Thiolutin as a novel therapeutic approach for esophageal squamous cell carcinoma through facilitating SNAIL degradation.; Authors - Chao Jing
human	WB	Clin Transl Med; MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma.; Authors - Mengqing Xiao ; KD Validated
human	WB	Oncogene; The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.; Authors - Sparks A A