Anticorps Polyclonal de lapin anti-PSMC4

PSMC4 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 11389-1-AP

Synonymes

MB67 interacting protein, MIP224, PSMC4, S6, Tat binding protein 7, TBP 7, TBP7

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Galerie de données de validation

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  WB analysis using 11389-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11389-1-AP (PSMC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11389-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11389-1-AP (PSMC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11389-1-AP

  Immunofluorescent analysis of HepG2 cells, using PSMC4 antibody 11389-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of SH-SY5Y using 11389-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PSMC4 antibody (11389-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11389-1-AP cible PSMC4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMC4 Protéine recombinante Ag1943
• Nom complet: proteasome (prosome, macropain) 26S subunit, ATPase, 4
• Masse moléculaire calculée: 418 aa, 47 kDa
• Poids moléculaire observé: 43-50 kDa
• Numéro d’acquisition GenBank: BC014488
• Symbole du gène: PSMC4
• Identification du gène (NCBI): 5704
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMC4(proteasome (prosome, macropain) 26S subunit, ATPase, 4) is also named as TBP7, S6, TBP7, MIP224 and belongs to the AAA ATPase family. The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The predicted 458-amino acid TBP7 protein is 57% identical to TBP1 and they can form heterodimers(PMID:8419915).

Publications

Species	Application	Title
mouse	IF	Neuron; Antibody Therapy Targeting RAN Proteins Rescues C9 ALS/FTD Phenotypes in C9orf72 Mouse Model.; Authors - Lien Nguyen
human	WB	Nat Commun; The 20S as a stand-alone proteasome in cells can degrade the ubiquitin tag.; Authors - Indrajit Sahu
rat	WB	Nutrients; Role of Epigallocatechin Gallate in Glucose, Lipid, and Protein Metabolism and L-Theanine in the Metabolism-Regulatory Effects of Epigallocatechin Gallate.; Authors - Ling Lin
human	IHC	Biosci Rep; A novel role of proteasomal β1 subunit in tumorigenesis.; Authors - Yuan Fuqiang F
rat	WB	Cells; The Prostaglandin E2 Receptor EP4 Promotes Vascular Neointimal Hyperplasia through Translational Control of Tenascin C via the cAPM/PKA/mTORC1/rpS6 Pathway; Authors - Hu Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

Was tested in ICC on PFA-fixed human cells. Provides a signal that looks specific (as compared to no primary control). 4% PFA followed by methanol permebealisation; antibody prepared in 5% goat serum in PBST, overnight incubation (no blocking step).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, SH-SY5Y cells

PSMC4 Antibody Immunofluorescence validation (1:1000 dilution) in Human, SH-SY5Y cells (Cat no:11389-1-AP)