Anticorps Monoclonal anti-PSMB8

PSMB8 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

2A5B6

N° de cat : CL594-66759

Synonymes

beta5i, D6S216, D6S216E, LMP7, Low molecular mass protein 7, Macropain subunit C13, Proteasome component C13, Proteasome subunit beta 5i, Proteasome subunit beta type 8, PSMB5i, PSMB8, RING10, Y2

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Galerie de données de validation

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  IF Staining of C2C12 using CL594-66759

  N/A

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  IF Staining of C2C12 using CL594-66759

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CoraLite®594 PSMB8 antibody (CL594-66759, Clone: 2A5B6 ) at dilution of 1:200, CL488-phalloidin (blue).

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  IF Staining of human liver cancer using CL594-66759

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 PSMB8 antibody (CL594-66759, Clone: 2A5B6 ) at dilution of 1:200.

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  IF Staining of human liver cancer using CL594-66759

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 PSMB8 antibody (CL594-66759, Clone: 2A5B6 ) at dilution of 1:200.

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  IF Staining of HeLa using CL594-66759

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PSMB8 antibody (CL594-66759, Clone: 2A5B6 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-66759

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PSMB8 (CL594-66759, Clone:2A5B6) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF: cellules C2C12, cellules HeLa, tissu de cancer du foie humain
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66759 cible PSMB8 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PSMB8 Protéine recombinante Ag6780
• Nom complet: proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC001114
• Symbole du gène: PSMB8
• Identification du gène (NCBI): 5696
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).