Anticorps Polyclonal de lapin anti-PSMB1

PSMB1 Polyclonal Antibody for WB, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

15

Avis (1)

N° de cat : 11749-1-AP

Synonymes

20S proteasome, HC5, KIAA1838, Macropain subunit C5, PMSB1, Proteasome component C5, Proteasome gamma chain, Proteasome subunit beta type 1, PSC5, PSMB1

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Galerie de données de validation

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  WB analysis using 11749-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11749-1-AP (PSMB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11749-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11749-1-AP (PSMB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11749-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11749-1-AP (PSMB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11749-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11749-1-AP (PSMB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 11749-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PSMB1 antibody (11749-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 11749-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PSMB1 (11749-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, cellules HepG2, cellules NIH/3T3, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,
• Résultats positifs en FC (Intra): cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

11749-1-AP cible PSMB1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMB1 Protéine recombinante Ag2345
• Nom complet: proteasome (prosome, macropain) subunit, beta type, 1
• Masse moléculaire calculée: 241 aa, 26 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC020807
• Symbole du gène: PSMB1
• Identification du gène (NCBI): 5689
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMB1(Proteasome subunit beta type-1) is also named as PSC5 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The gene encodes a 241 amino acid protein with a 28 amino acid propeptide and two glycosylation sites.

Publications

Species	Application	Title
human	WB	EMBO J; Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins.; Authors - Guiyou Tian
human	IF	Cancer Lett; Combinations of ivermectin with proteasome inhibitors induce synergistic lethality in multiple myeloma; Authors - Hongmei Luo
mouse	WB,IHC	J Proteome Res; Novel breast cancer metastasis-associated proteins.; Authors - Ho Jiapei J
mouse	WB	Front Pharmacol; Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy.; Authors - Xin Xie
rat	WB	Cardiovasc Drugs Ther; Quercetin Prevents In Vivo and In Vitro Myocardial Hypertrophy Through the Proteasome-GSK-3 Pathway.; Authors - Kuixiang Chen
rat	WB	PLoS One; Proteomic Analysis Reveals that Proteasome Subunit Beta 6 Is Involved in Hypoxia-Induced Pulmonary Vascular Remodeling in Rats.; Authors - Wang Jian J

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Janelle (Verified Customer) (09-12-2022)

Specific to target

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000