Anticorps Polyclonal de lapin anti-PSIP1

• Phare
• Validé par KD/KO

PSIP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (2)

N° de cat : 25504-1-AP

Synonymes

CLL associated antigen KW 7, DFS 70, DFS70, LEDGF, p52, p75, PAIP, PSIP1, PSIP2

---

Galerie de données de validation

• 

  WB analysis using 25504-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25504-1-AP (PSIP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 25504-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25504-1-AP (PSIP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25504-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25504-1-AP (PSIP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 25504-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 25504-1-AP (PSIP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 25504-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 25504-1-AP (PSIP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 25504-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25504-1-AP (PSIP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 25504-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25504-1-AP (PSIP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 25504-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSIP1 antibody (25504-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

25504-1-AP cible PSIP1 dans les applications de WB, RIP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSIP1 Protéine recombinante Ag21886
• Nom complet: PC4 and SFRS1 interacting protein 1
• Masse moléculaire calculée: 530 aa, 60 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC064135
• Symbole du gène: PSIP1
• Identification du gène (NCBI): 11168
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSIP1 is a chromatin associated protein that is shown to act as a transcriptional coactivator as well as an RNA-binding protein. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.

Publications

Species	Application	Title
human	WB	Sci Adv; The H3K36me2 writer-reader dependency in H3K27M-DIPG.; Authors - Jia-Ray Yu ; KD Validated
human	WB	Sci Adv; LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cells.; Authors - Gary LeRoy ; KO Validated
human	RIP	Elife; The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.; Authors - Belinda Baquero-Perez

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Christopher (Verified Customer) (03-03-2022)

worked very well with 1 hour incubation at room temperature

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HT1080

PSIP1 Antibody Western Blot validation (1:2000 dilution) in HT1080 (Cat no:25504-1-AP)

FH

Siting (Verified Customer) (01-28-2020)

It worked perfectly in my mouse heart tissue extract for western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue