Anticorps Polyclonal de lapin anti-PSEN2-Specific

• Phare
• Validé par KD/KO

PSEN2-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 16168-1-AP

Synonymes

AD3L, AD3LP, AD4, AD5, E5 1, Presenilin 2, PS 2, PS2, PSEN2, PSEN2-Specific, PSNL2, STM 2, STM2

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Galerie de données de validation

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  WB analysis of A549 using 16168-1-AP

  WB result of PSEN2-Specific antibody (16168-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSEN2-Specific transfected A549 cells.

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  WB analysis using 16168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16168-1-AP (PSEN2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16168-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 16168-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 16168-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 16168-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 16168-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16168-1-AP (PSEN2-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 16168-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16168-1-AP (PSEN2-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 16168-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16168-1-AP (PSEN2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 16168-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 16168-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules A549, cellules NIH/3T3
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral de souris, tissu de cancer du sein humain, tissu de côlon humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:800
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

16168-1-AP cible PSEN2-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: presenilin 2 (Alzheimer disease 4)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: NM_000447
• Symbole du gène: PSEN2
• Identification du gène (NCBI): 5664
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Presenilins can regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. These transmembrane proteins are considered to be involved in early onset of Alzheimer's disease. Presenilin 2 mainly localizes in large neurons. It exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (about 23 kDa) fragments.This antibody is specific to presenilin 2. It will not bind presenilin 1.

Publications

Species	Application	Title
mouse	WB	Gut Microbes; Gut-derived β-amyloid: Likely a centerpiece of the gut-brain axis contributing to Alzheimer's pathogenesis; Authors - Jinghua Jin