Anticorps Polyclonal de lapin anti-Presenilin-1-Specific

• Phare
• Validé par KD/KO

Presenilin-1-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (1)

N° de cat : 16163-1-AP

Synonymes

Presenilin-1-Specific, PSEN1, PSNL1, PS1, PS-1, AD3, EC:3.4.23.-, Presenilin-1, Presenilin-1 CTF subunit, Presenilin-1 CTF12, Presenilin-1 NTF subunit

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Galerie de données de validation

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  WB analysis using 16163-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 16163-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16163-1-AP

  IP result of anti-Presenilin-1-Specific (IP:16163-1-AP, 4ug; Detection:16163-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human prostate cancer using 16163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16163-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 16163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules COS-7, cellules HeLa, cellules HepG2, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris, tissu rénal humain
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 1 publications below

Informations sur le produit

16163-1-AP cible Presenilin-1-Specific dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: presenilin 1
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 21 kDa, 53 kDa
• Numéro d’acquisition GenBank: NM_000021
• Symbole du gène: PSEN1
• Identification du gène (NCBI): 5663
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome (PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein.

Publications

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression; Authors - Rui Zhou
human	WB	Brain; MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.; Authors - Bing-Lin Zhu
human,mouse	WB	Cell Rep; CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer's Disease.; Authors - Yang-Ping Shentu
mouse	WB	J Neuroinflammation; Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer's disease-like mice.; Authors - Qingyong Li
human	WB	Cell Commun Signal; Presenilin1 exerts antiproliferative effects by repressing the Wnt/β-catenin pathway in glioblastoma.; Authors - Wei Yang ; KD Validated
mouse	WB	Mol Neurobiol; Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.; Authors - Yasuteru Inoue

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaoyu (Verified Customer) (02-07-2024)

Good signal

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: brain

Presenilin-1-Specific Antibody Western Blot validation (1:1000 dilution) in brain (Cat no:16163-1-AP)