Anticorps Polyclonal de lapin anti-PRSS8

PRSS8 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 15527-1-AP

Synonymes

CAP1, PROSTASIN, protease, serine, 8, PRSS8, Serine protease 8

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Galerie de données de validation

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  WB analysis of human colon using 15527-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 15527-1-AP (PRSS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15527-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15527-1-AP (PRSS8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 15527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15527-1-AP (PRSS8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15527-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15527-1-AP (PRSS8 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 15527-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15527-1-AP (PRSS8 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de côlon humain, tissu rénal de souris
• Résultats positifs en IHC: tissu ovarien de souris, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 5 publications below

Informations sur le produit

15527-1-AP cible PRSS8 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRSS8 Protéine recombinante Ag7234
• Nom complet: protease, serine, 8
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC001462
• Symbole du gène: PRSS8
• Identification du gène (NCBI): 5652
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protease serine S1 family member 8 (PRSS8), a membrane-anchored serine protease, has been reported to be involved in the development of several human cancers. PRSS8 protein level was highly expressed in well differentiated cancer cell and was lower or absent in poorly differentiated cancer cell. Significantly higher levels of prostasin were found in early stage OVC serum samples compared to benign ovarian and normal donor samples. Overexpression of PRSS8 mRNA and high levels of prostasin in multiple subtypes of early stage ovarian tumors may provide clinical biomarkers for early detection of OVC. (PMID: 27036110, PMID: 27050145)

Publications

Species	Application	Title
mouse	WB,IHC	Acta Physiol (Oxf); Zymogen-locked mutant prostasin (Prss8) leads to incomplete proteolytic activation of the epithelial sodium channel (ENaC) and severely compromises triamterene tolerance in mice.; Authors - Daniel Essigke
mouse	WB	Br J Pharmacol; PMID: 31222723; Authors - Ming-Ming Wu
human	WB,IHC	J Hypertens; Prostasin and matriptase (ST14) in placenta from preeclamptic and healthy pregnant women.; Authors - Britta Frederiksen-Møller
human	WB,IHC	Acta Physiol (Oxf); Apical serine protease activity is necessary for assembly of a high-resistance renal collecting duct epithelium.; Authors - Steensgaard M M
human	WB	Pflugers Arch; The epithelial Na+ channel α- and γ-subunits are cleaved at predicted furin-cleavage sites, glycosylated and membrane associated in human kidney.; Authors - Rikke Zachar
human	WB	Nephrol Dial Transplant; PMID: 25609736; Authors - Henrik Andersen