Anticorps Polyclonal de lapin anti-PRR5

PRR5 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 17948-1-AP

Synonymes

FLJ20185k, PP610, proline rich 5 (renal), Proline rich protein 5, Protein observed with Rictor 1, Protor 1, PROTOR1, PRR5

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Galerie de données de validation

• 

  WB analysis of human kidney using 17948-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17948-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17948-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 17948-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17948-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17948-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17948-1-AP

  IP result of anti-PRR5 (IP:17948-1-AP, 3ug; Detection:17948-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse testis using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17948-1-AP (PRR5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17948-1-AP (PRR5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse kidney using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17948-1-AP

  Immunofluorescent analysis of HepG2 cells, using PRR5 antibody 17948-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu rénal humain, cellules COLO 320, cellules HEK-293, cellules HepG2, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire de souris, tissu cérébral de souris, tissu placentaire humain, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

17948-1-AP cible PRR5 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRR5 Protéine recombinante Ag12424
• Nom complet: proline rich 5 (renal)
• Masse moléculaire calculée: 31 kDa, 43 kDa
• Poids moléculaire observé: 33 kDa, 50 kDa
• Numéro d’acquisition GenBank: BC016921
• Symbole du gène: PRR5
• Identification du gène (NCBI): 55615
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRR5, also named as PROTOR1 and PP610, is a subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. It acts as a tumor suppressor in breast cancer. PRR5 has some isoforms with MW 31-33 kDa and 41-50 kDa.

Publications

Species	Application	Title
human	IHC	Nat Cell Biol; m6A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer.; Authors - Jun Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tominica (Verified Customer) (08-19-2019)

Rabbit polyclonal anti-FUS/TLS Ab-TLS-M generated against amino acids 260–274 of mouse FUS/TLS, a stretch where the sequence is identical to the human FUS/TLS sequence. works very well.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: human motor neuron