Anticorps Polyclonal de lapin anti-Peripherin

Peripherin Polyclonal Antibody for WB, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 17399-1-AP

Synonymes

peripherin, PRPH, NEF4

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Galerie de données de validation

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  WB analysis using 17399-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin antibody) at dilution of 1:120000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 17399-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 17399-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 17399-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of rat brain using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17399-1-AP (PRPH antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17399-1-AP (PRPH antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of human colon using 17399-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon using 17399-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of PC-12 using 17399-1-AP

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (17399-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, cellules COLO 320, tissu de côlon de rat, tissu de côlon humain, tissu de moelle épinière de rat
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon humain,
• Résultats positifs en FC (Intra): cellules PC-12
• Résultats positifs en cytométrie: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

17399-1-AP cible Peripherin dans les applications de WB, IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peripherin Protéine recombinante Ag11365
• Nom complet: peripherin
• Masse moléculaire calculée: 470 aa, 54 kDa
• Poids moléculaire observé: 54-60 kDa
• Numéro d’acquisition GenBank: BC032703
• Symbole du gène: Peripherin
• Identification du gène (NCBI): 5630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes.

Publications

Species	Application	Title
rat	IF	Biotechniques; Antibody incubation at 37°C improves fluorescent immunolabeling in free-floating thick tissue sections.; Authors - Xia Xiao