Anticorps Polyclonal de lapin anti-PRPF8

PRPF8 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 11171-1-AP

Synonymes

HPRP8, p220, PRP8, PRP8 homolog, PRPC8, PRPF8, RP13, Splicing factor Prp8

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Galerie de données de validation

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  WB analysis of Jurkat using 11171-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11171-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11171-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11171-1-AP

  IP result of anti-PRPF8 (IP:11171-1-AP, 4ug; Detection:11171-1-AP 1:500) with HeLa cells lysate 2000ug.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules K-562, tissu placentaire humain
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

11171-1-AP cible PRPF8 dans les applications de WB, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRPF8 Protéine recombinante Ag1652
• Nom complet: PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
• Masse moléculaire calculée: 274 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC064370
• Symbole du gène: PRPF8
• Identification du gène (NCBI): 10594
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRPF8, a 220kd protein,is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursor. It has a central structural role in the architecture of the U5 small nuclear ribonucleoprotein (snRNP) particle.

Publications

Species	Application	Title
human	WB	Mol Cell; Mechanism of exon ligation by human spliceosome.; Authors - Xiechao Zhan
human	WB	Genes Dev; An integrative analysis of colon cancer identifies an essential function for PRPF6 in tumor growth.; Authors - Adam S Adler
human	WB	Nucleic Acids Res; Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.; Authors - Misbah Khan
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou
human	WB	RNA; Rearrangements within human spliceosomes captured after exon ligation.; Authors - Ilagan Janine O JO
human	IP	J Biol Chem; Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.; Authors - Zhe Chen