Anticorps Polyclonal de lapin anti-PRPF6

• Phare
• Validé par KD/KO

PRPF6 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 23929-1-AP

Synonymes

ANT 1, C20orf14, hPrp6, Pre mRNA processing factor 6, PRP6 homolog, PRPF6, TOM, U5 102 kDa protein, U5 102K

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Galerie de données de validation

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  WB analysis of HEK-293 using 23929-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23929-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23929-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 23929-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 23929-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23929-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 23929-1-AP

  IP result of anti-PRPF6 (IP:23929-1-AP, 4ug; Detection:23929-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IHC staining of human testis using 23929-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23929-1-AP (PRPF6 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 23929-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23929-1-AP (PRPF6 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

23929-1-AP cible PRPF6 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRPF6 Protéine recombinante Ag13817
• Nom complet: PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae)
• Masse moléculaire calculée: 941 aa, 107 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC001666
• Symbole du gène: PRPF6
• Identification du gène (NCBI): 24148
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRPF6 is involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, it can enhance dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.

Publications

Species	Application	Title
human	WB	Hum Genet; A CRISPR and high-content imaging assay compliant with ACMG/AMP guidelines for clinical variant interpretation in ciliopathies.; Authors - Liliya Nazlamova
mouse	WB,IF,IHC	Cancer Sci; Splicing factor PRPF6 upregulates oncogenic androgen receptor signaling pathway in hepatocellular carcinoma.; Authors - Huijuan Song ; KD Validated
human	WB	Int J Biol Sci; PRPF6 promotes androgen receptor/androgen receptor-variant 7 actions in castration-resistant prostate cancer cells.; Authors - Wei Liu ; KD Validated
human,mouse	IF,WB	Front Genet; Microtubule modification defects underlie cilium degeneration in cell models of retinitis pigmentosa associated with pre-mRNA splicing factor mutations; Authors - Liliya Nazlamova