Anticorps Polyclonal de lapin anti-PRPF4

PRPF4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10728-1-AP

Synonymes

HPRP4, HPRP4P, PRP4, PRP4 homolog, Prp4p, PRPF4, U4/U6 snRNP 60 kDa protein, WD splicing factor Prp4

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Galerie de données de validation

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  WB analysis using 10728-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10728-1-AP (PRPF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10728-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10728-1-AP (PRPF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10728-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10728-1-AP (PRPF4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10728-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10728-1-AP (PRPF4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10728-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10728-1-AP (PRPF4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10728-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10728-1-AP (PRPF4 antibody) at dilution of 1:10 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules K-562, cellules Raji
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

10728-1-AP cible PRPF4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRPF4 Protéine recombinante Ag1193
• Nom complet: PRP4 pre-mRNA processing factor 4 homolog (yeast)
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC001588
• Symbole du gène: PRPF4
• Identification du gène (NCBI): 9128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRPF4, also named as U4/U6 small nuclear ribonucleoprotein Prp4, is a 522 amino acid protein, which contains seven WD repeats. PRPF4 localizes in the Nucleus speckle. PRPF4 participates in pre-mRNA splicing and is a part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.PRPF4-mediated phosphorylation of KLF13 plays a role in the regulation of CCL5 expression in T lymphocytes

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
human	WB	Hum Mol Genet; PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa.; Authors - Tanackovic Goranka G
mouse	WB	J Biol Chem; Intercellular transfer of proteins as identified by stable isotope labeling of amino acids in cell culture.; Authors - Li Ming M