Anticorps Polyclonal de lapin anti-Prokineticin 1

Prokineticin 1 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

N° de cat : 15152-1-AP

Synonymes

PROK1, Mambakine, Endocrine-gland-derived vascular endothelial growth factor, EG-VEGF, EGVEGF

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Galerie de données de validation

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  IHC staining of human colon cancer using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 15152-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Prokineticin 1 antibody (15152-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of NIH/3T3 using 15152-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human Prokineticin 1 (15152-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu hépatique de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3,
• Résultats positifs en FC (Intra): cellules NIH/3T3,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15152-1-AP cible Prokineticin 1 dans les applications de IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Prokineticin 1 Protéine recombinante Ag5098
• Nom complet: prokineticin 1
• Masse moléculaire calculée: 12 kDa
• Numéro d’acquisition GenBank: BC025399
• Symbole du gène: Prokineticin 1
• Identification du gène (NCBI): 84432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Prokineticin 1 (PROK1) is also named as EG-VEGF and Mambakine, belongs to the to the AVIT (prokineticin) family. Prokineticin signaling comprises two secreted proteins (Prok-1 and Prok-2) and two cognate G-protein coupled receptors (PK-R1 and PK-R2) that are widely expressed in different tissues and of great versatility. Prokineticins were shown to promote angiogenesis in steroidgenic glands, heart and reproductive organs (PMID:18440852). PROK1 has been described as a secretory protein with pleiotropic functions and as a novel tissue-specific angiogenic factor (PMID:32355954). EG-VEGF/PK-1, described as selective angiogenic mitogen, is widely expressed in different tissues including steroidogenic endocrine glands (PMID:16320832). A lot of data suggests EG-VEGF to be restricted to endocrine glands and to some endocrine-dependent organs (PMID:28386275).