Anticorps Polyclonal de lapin anti-PRKG1

• Phare
• Validé par KD/KO

PRKG1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 21646-1-AP

Synonymes

cGK 1, cGK1, CGKI, cGKI alpha, cGKI BETA, PGK, PKG, PRKG1, PRKG1B, PRKGR1A, PRKGR1B, Protein Kinase G1 alpha

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Galerie de données de validation

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  WB analysis using 21646-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21646-1-AP (PRKG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21646-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21646-1-AP (PRKG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21646-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21646-1-AP (PRKG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21646-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21646-1-AP (PRKG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21646-1-AP

  IP result of anti-PRKG1 (IP:21646-1-AP, 4ug; Detection:21646-1-AP 1:600) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 21646-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21646-1-AP (PRKG1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 21646-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21646-1-AP (PRKG1 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cardiaque humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 2 publications below

Informations sur le produit

21646-1-AP cible PRKG1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRKG1 Protéine recombinante Ag16333
• Nom complet: protein kinase, cGMP-dependent, type I
• Masse moléculaire calculée: 686 aa, 78 kDa
• Poids moléculaire observé: 65-78 kDa
• Numéro d’acquisition GenBank: BC127090
• Symbole du gène: PRKG1
• Identification du gène (NCBI): 5592
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRKG1(cGMP-dependent protein kinase 1) is also named as cGK1, PRKG1B, PRKGR1A, PRKGR1B and belongs to the cGMP subfamily. It serves as an integral component of second messenger signaling in a number of biological contexts including cell differentiation, memory, and vasodilation(PMID:21893290). Its activity prevents pathological-level nitric oxide-induced apoptosis and promotes DNA synthesis/cell proliferation in vascular smooth muscle cells(PMID:20060325). PRKG1 has 2 isoforms produced by alternative splicing with the molecular weight of 76 kDa and 78 kDa. The autophosphorylation can increase the kinase activity and it can form a monomer with the molecular weight of 65 kDa that is produced by proteolytic cleavage. SDS-PAGE revealed that proteolysis generated two different monomers with molecular masses of 70 and 68 kDa of CGK1-beta(PMID:8702828).

Publications

Species	Application	Title
rat	WB	Environ Pollut; Fluoride-induced unrestored arrest during haploid period of spermatogenesis via the regulation of DDX25 in rats.; Authors - Yongli Han
mouse	WB	Clin Sci (Lond); Empagliflozin prevents cardiomyopathy via sGC-cGMP-PKG pathway in type 2 diabetes mice.; Authors - Mei Xue
bovine	WB	Front Cell Dev Biol; Myostatin Mutation Promotes Glycolysis by Increasing Phosphorylation of Phosphofructokinase via Activation of PDE5A-cGMP-PKG in Cattle Heart.; Authors - Mingjuan Gu ; KD Validated
rat	WB	Front Pharmacol; Emodin Interferes With Nitroglycerin-Induced Migraine in Rats Through CGMP-PKG Pathway.; Authors - Shuding Sun
human	WB	Cancer Cell Int; NEK7 promotes gastric cancer progression as a cell proliferation regulator.; Authors - Yi-Ke Li
rat	WB	Eur J Pharmacol; A novel phosphodiesterase 9A inhibitor LW33 protects against ischemic stroke through the cGMP/PKG/CREB pathway.; Authors - Jia-Yi You